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Endeavorsil^® Columns

Endeavorsil^® Columns

Features of Endeavorsil™ Columns

Combined speed, resolution and sensitivity
Reduced analysis time and solvent waste
High efficiency combined with high selectivity generates more information and productivity
Superior column performance at higher pressure
Excellent separation characteristics over wide pH range

Endeavorsil™ Material Characteristics

Speed

Column:	Listed on chromatograms
Mobile Phase:	MeCN:H₂O = 55:45
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Uracil	4. Toluene
	2. Acetophenone	5. Naphthalene
	3. Methyl benzoate

Selectivity*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeCN:H₂O = 60:40
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Uracil	5. Butylbenzene
	2. Caffeine	6. o-Terphenyl
	3. Phenol	7. Triphenylene
	4. Toluene	8. Amylbenzene

Separation of Hydrophobic, Polar and Basic Mixture*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:20 mM phosphate buffer (pH 7) = 80:20
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Uracil	4. Naphthalene
	2. Butyl paraben	5. Acenaphthene
	3. Dipropyl phthalate	6. Amitriptyline

Strong Basic Compounds at Neutral pH

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:20 mM phosphate buffer (pH 7) = 65:35
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Pyridine	4. Nortriptyline
	2. Codeine	5. Diphenhydramine
	3. Quinine

Cold Medicines*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 35:65
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 220 nm
Sample:	1. Doxylamine	4. Acetylsalicylic acid
	2. Acetaminophen	5. Salicylic acid
	3. Acetanilide

Water-Soluble Vitamins

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	10 mM NH₄COOH, pH 3
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Pyridoxamine	3. Pyridoxal
	2. L-Ascorbic acid	4. Nicotinamide

Galushko Test*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:H₂O = 50:50
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Uracil	4. Benzene
	2. Aniline	5. Toluene
	3. Phenol

Verzele Test

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:0.5% CH₃COONa (pH 7.8) = 60:40
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Acetylacetone
	2. 1-Nitronaphthalene
	3. Naphthalene

Parabens*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeCN:20 mM K₂HPO₄ (pH 7) = 50:50
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Methyl paraben	3. Propyl paraben
	2. Ethyl paraben	4. Butyl paraben

Catecholamines

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% TFA in H₂O
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 270 nm
Sample:	1. Norepinephrine
	2. Epinephrine
	3. Dopamine

β -Blockers at Neutral PH*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeCN:20 mM phosphate buffer (pH 7) = 30:70
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 220 nm
Sample:	1. Nadolol	4. Labetolol
	2. Pindolol	5. Propranolol
	3. Metoprolol

β -Blockers at Low PH

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% TFA in MeCN:0.1% TFA in H₂O = 25:75
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 220 nm
Sample:	1. Nadolol	5. Labetolol
	2. Pindolol	6. Propranolol
	3. Acebutolol	7. Alprenolol
	4. Metoprolol

TCAs at Low PH*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% TFA in MeCN:0.1% TFA in H₂O = 35:65
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Doxepin	4. Amitriptyline
	2. Desipramine	5. Trimipramine
	3. Nortriptyline	6. Clomipramine

Antiulcers

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:10 mM CH₃COONH₄ (pH 7) = 35:65
Flow Rate:	0.5 mL/min
Temperature:	30 ºC
Detection:	UV 220 nm
Sample:	1. Famotidine	3. Cimetidine
	2. Ranitidine	4. Nizatidine

Polar Acids*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 20:80
Flow Rate:	0.5 mL/min
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. p-Aminobenzoic acid	4. Salicylic acid
	2. Homovanillic acid	5. p-Chlorobenzoic acid
	3. Sorbic acid	6. p-Nitrobenzoic acid

Acidic Compounds

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 25:75
Flow Rate:	0.5 mL/min
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. L-Ascorbic acid	4. Acetylsalicylic acid
	2. p-Aminobenzoic acid	5. Sorbic acid
	3. Homovanillic acid	6. Salicylic acid

Anti-inflammatories*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 50:50
Flow Rate:	0.5 mL/min
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. Phenacetin	4. Fenoprofen
	2. Tolmetin	5. Flurbiprofen
	3. Ketoprofen	6. Ibuprofen

Antibacterials

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 20:80
Flow Rate:	0.5 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Sulfanilamide	6. Sulfamethoxazole
	2. Carbadox	7. Sulfisoxazole
	3. Sulfamerazine	8. Oxolinic acid
	4. Sulfamethoxypyridazine	9. Sulfadimethoxine
	5. Furazolidone	10. Sulfaquinoxaline

Antifungals*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 30:70
Flow Rate:	0.5 mL/min
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. p-Aminobenzoic acid	3. Benzoic acid
	2. Acetylsalicylic acid	4. Salicylic acid

Steroids

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:H₂O = 50:50
Flow Rate:	0.5 mL/min
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. Prednisone	4. Hydrocortisone 21-acetate
	2. Prednisolone	5. 11-α -Hydroprogesterone
	3. Dexamethasone

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