Leapsil™ Columns

Features of Leapsil™ Columns

• Ultra fast separation without compromising resolution
• Compatible with all HPLC and UHPLC instruments
• Method development flexibility
• Wide pH stability
• Outstanding selectivity

Leapsil™ Material Characteristics

Speed

Column:	Leapsil™ 2.7 μm C18, 50 x 2.1 mm
Cat. No.:	86004
Mobile Phase:	MeCN:H₂O = 60:40
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. Uracil	4. Toluene
	2. Acetophenone	5. Naphthalene
	3. Methyl benzoate

Selectivity*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:H₂O = 45:55
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. Thiourea	5. N,N-Dimethylaniline
	2. Aniline	6. Ethyl benzoate
	3. Phenol	7. Toluene
	4. o,m,p-Toluidine	8. Ethylbenzene

Galushko Test*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:H₂O = 50:50
Flow Rate:	0.3 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Uracil	4. Benzene
	2. Aniline	5. Toluene
	3. Phenol

Steroids

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeOH:H₂O = 55:45
Flow Rate:	0.3 mL/min
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. Prednisone	4. Cortisone 21-acetate
	2. Prednisolone	5. 11-α -Hydroprogesterone
	3. Betamethasone

β -Blockers at Neutral pH*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	MeCN:20 mM phosphate buffer (pH 7) = 25:75
Flow Rate:	0.3 mL/min
Temperature:	30 ºC
Detection:	UV 220 nm
Sample:	1. Nadolol	4. Labetolol
	2. Pindolol	5. Propranolol
	3. Metoprolol

β-Blockers at Low pH

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% TFA in MeCN:0.1% TFA in H₂O = 25:75
Flow Rate:	0.3 mL/min
Temperature:	30 ºC
Detection:	UV 220 nm
Sample:	1. Nadolol	4. Metoprolol
	2. Pindolol	5. Labetolol
	3. Acebutolol	6. Propranolol

TCAs at Low pH*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% TFA in MeCN:0.1% TFA in H₂O = 40:60
Flow Rate:	0.3 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Doxepin	4. Amitriptyline
	2. Protriptyline	5. Trimipramine
	3. Nortriptyline	6. Clomipramine

Polar Acids

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 25:75
Flow Rate:	0.3 mL/min
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. p-Aminobenzoic acid	4. Salicylic acid
	2. Homovanillic acid	5. p-Nitrobenzoic acid
	3. Sorbic acid	6. p-Toluic acid

Acidic Compounds*

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 25:75
Flow Rate:	0.3 mL/min
Temperature:	30 ºC
Detection:	UV 254 nm
Sample:	1. L-Ascorbic acid	4. Acetylsalicylic acid
	2. p-Aminobenzoic acid	5. Sorbic acid
	3. Homovanillic acid	6. Salicylic acid

Antibacterials

Column:	Listed on chromatograms
Dimension:	50 x 2.1 mm
Mobile Phase:	0.1% HCOOH in MeCN:0.1% HCOOH in H₂O = 20:80
Flow Rate:	0.3 mL/min
Temperature:	Ambient
Detection:	UV 254 nm
Sample:	1. Sulfanilamide	6. Furazolidone
	2. Carbadox	7. Sulfamethoxazole
	3. Sulfamerazine	8. Sulfisoxazole
	4. Sulfamethazine	9. Oxolinic acid
	5. Sulfamethoxypyridazine	10. Sulfadimethoxine