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UHPLC-MSMS Determination of Five Steroid Hormone Drugs

Method: HPLC-MS
Matrix: Medicine
Application No.: 101974
Componds: Hydrocortisone, Estradiol, Ethinylestradiol, Equilin, Estrone
Columns: Navigatorsil 2.7 μm C18, 100 x 2.1 mm
Cat No.: 88003
Sample Pretreatment: Samples: Individual standard stock solution was made by acetonitrile at 1 mg/mL.
Conditions: Mobile Phase:
A: 1 mM ammonium fluoride
B: acetonitrile
Gradient: hold 10% B for 4 min, 10% to 40% B in 3 min, 40 to 60% B in 4 min, hold 1 min, 60 to 10% B in 1 min, equilibrate at 10% B for 4 min
Ionization mode: ESI-
Flow Rate: 0.2 mL/min
Injection: 2 µL
Temperature: 25 ℃
Publisher: Dikma Technologies Inc.
Keyword: Hydrocortisone, Estradiol, Ethinylestradiol, Equilin, Estrone, UHPLC-MSMS, Navigatorsil C18
Abstract: All compounds were separated by a gradient procedure on a DIKMA Navigatorsil C18 column within 12 min.

 

 

No.

Compound

Concentration (µg/mL)

Retention Time(min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

1

Hydrocortisone

0.2

8.48

361.2 → 331.1

361.2 → 297.1

2

Estradiol

0.2

10.32

271.2 → 145.2

271.2 → 183.2

3

Ethinylestradiol

0.2

10.92

295.1 → 145.1

295.1 → 159.1

4

Equilin

0.2

11.05

267.1 → 143.1

267.1 → 115.0

5

Estrone

0.2

11.23

269.2 → 145.1

269.2 → 159.1