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HPLC Analysis of 6 Local Anesthetics on Diamonsil Plus C18 Column

Method: HPLC
Matrix: Medicine
Application No.: 101968
Componds: Procainamide hydrochloride, Procaine hydrochloride, Tetracaine, ydrochloride, Lidocaine, Bupivacaine, Pramocaine
Columns: Diamonsil® Plus C18 5μm 250 x 4.6 mm
Cat No.: 99403
Sample Pretreatment: Standards: Individual stock solutions were prepared in methanol at a concentration of 1.0 mg/mL. A working solution of the mixture of all 6 drugs was obtained by dilution of the stock solution with mobile phase.
Conditions: Mobile Phase:
A: 30mM Potassium Phosphate Dibasic (pH6.2)
B: Acetonitrile: Methanol = 4: 6
Gradient: hold 30% B for 0.5 min, 30 to 60% B in 1.5 min, 60 to 70% B in 5 min, hold 70% B for 11 min, 70 to 30% B in 1 min, equilibrate at 70% B for 6 min.
Flow Rate: 1.0 mL/min
Injection: 10 µL
Temperature: 30 ℃
Wavelength: 220 nm
Publisher: Dikma Technologies Inc.
Keyword: Procainamide hydrochloride, Procaine hydrochloride, Tetracaine, ydrochloride, Lidocaine, Bupivacaine, Pramocaine, HPLC, Diamonsil Plus C18
Abstract: Chromatography was done on a DIKMA Diamonsil Plus column embedded with C18 stationary phase using a gradient elution procedure.

 

No.

Compound

Concentration (µg/mL)

Retention Time(min)

Resolution

Asymmetry

1

Procainamide hydrochloride

20

3.404

-

1.15

2

Procaine hydrochloride

20

4.603

8.82

1.13

3

Tetracaine hydrochloride

20

10.765

37.01

1.17

4

Lidocaine

20

11.247

2.50

1.07

5

Bupivacaine

20

14.675

14.17

1.08

6

Pramocaine

20

16.579

6.20

1.10