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Ceftizoxime Sodium

Method: HPLC
Matrix: Medicine
Application No.: 101963
Componds: Ceftizoxime Sodium, Salicylic Acid
Columns: Diamonsil® Plus C18 5μm 150 x 4.6 mm
Cat No.: 99401
Sample Pretreatment: pH 7.0 buffer: Dissolve 3.63 g of monobasic potassium phosphate and 10.73 g of dibasic sodium phosphate in water to obtain 1000 mL of solution.
Salicylic acid solution: Dissolve 0.6 g of salicylic acid in 5 mL of methanol and dilute with pH 7.0 Buffer to obtain 100 mL of solution.
Ceftizoxime Sodium solution: Dissolve a suitable quantity of ceftizoxime Sodium, accurately weighted, in pH 7.0 Buffer to obtain a solution having a known concentration of 1 mg of ceftizoxime sodium per mL.
System suitability solution: Transfer 0.2 mL of Ceftizoxime Sodium solution and 0.5 mL of Salicylic acid solution, dilute with pH 7.0 Buffer to 10 mL, and mix. This solution contains about 0.02 mg of ceftizoxime sodium per mL.
Conditions: Mobile phase: Dissolve 1.42 g of citric acid monohydrate and 0.865 g of dibasic sodium phosphate in water to obtain 1000 mL of pH 3.6 buffer. Prepare a mixture of pH 3.6 buffer and acetonitrile (9: 1)
Detector: UV 254 nm
Flow rate: 1.8 mL/min
Injection volume: 7 µL
Column temp.: 30 ℃
Publisher: Dikma Technologies Inc.
Keyword: Ceftizoxime Sodium, Salicylic Acid, HPLC, Diamonsil Plus C18
Abstract: Ceftizoxime Sodium is the sodium salt form of ceftizoxime and a semi-synthetic, broad-spectrum, beta-lactamase resistant, third-generation cephalosporin. The experimental conditions follow guidelines from the USP43-NF38 monograph method.

 

Peaks

Compound

Retention Time

(min)

Resolution

Tailing Factor

Theoretical Plates

1

Ceftizoxime Sodium

2.373

-

1.04

42907

2

Salicylic Acid

5.036

17.12

1.02

73313