An HPLC Method for Simultaneous Analysis of 8 Cephalosporins

Method:	HPLC
Matrix:	Medicine
Application No.:	101957
Componds:	Cefadroxil, Cefepime hydrochloride, Cephalexin, Cefaclor, Cefazolin sodium, Cefoxitin sodium, Cefoperazone dihydrate, Ceftiofur hydrochloride
Columns:	Diamonsil® Plus C18 5μm 250 x 4.6 mm
Cat No.:	99403
Sample Pretreatment:	Standards: Individual stock solutions were prepared in methanol at a concentration of 1 mg/mL. A working solution of the mixture of all 8 drugs was obtained by dilution of the stock solution with mobile phase.
Conditions:	Mobile Phase: A: 70 mM ammonium acetate, pH 5.6 B: methanol: acetonitrile = 6:4 Gradient: hold 10% B for 1 min, 10 to 60% B in 15 min, 60 to 80% B in 2 min, hold 80% B for 2 min, 80 to 10% B in 1 min, equilibrate at 10% B for 4 min. Flow Rate: 1.0 mL/min Injection: 10 µL Temperature: 25 ℃
Publisher:	Dikma Technologies Inc.
Keyword:	Cefadroxil, Cefepime hydrochloride, Cephalexin, Cefaclor, Cefazolin sodium, Cefoxitin sodium, Cefoperazone dihydrate, Ceftiofur hydrochloride, Diamonsil Plus C18
Abstract:	Using a DIKMA Diamonsil Plus 1.8 µm C18 column, all compounds were clearly separated within 15 min with good peak shape. This method can be applied to a variety of routine drug screening and analysis workflows.