Lab Glassware Division 中文(Chinese)

Rapid LC/MS/MS Method for the Simultaneous Determination of Potential Medicines for COVID-19 with Endeavorsil C18 Column

Method: HPLC-MS
Matrix: Medicine
Application No.: 101939
Componds: Chloroquine phosphate, Favipiravir, Saquinavir, Remdesivir, Ritonavir, Lopinavir
Columns: Endeavorsil C18 1.8 μm 100 x 2.1 mm
Cat No.: 87003
Sample Pretreatment: Samples: Except Chloroquine Phosphate which was dissolved in mixed solution of methanol and water (1:1 v/v), other standard stock solutions were made by methanol, then a standard mixture of six analyteswas prepared with each standard stock solution of 1mg/mL.
Conditions: Mobile Phase: A: 0.1% Formic acid in water
B:0.1% Formic acid in acetonitrile
Gradient: 10%B to 70%Bin1.5min, 70 to 90%B in 4min, hold0.5min, 90 to 10%B in 0.5min, equilibrate at 10%B for 3.5 min
Scan mode: ESI+;
Flow Rate: 0.2mL/min;
Injection: 1µL;
Temperature: 30℃
Publisher: Dikma Technologies Inc.
Keyword: LC/MS/MS, COVID-19, Chloroquine phosphate, Favipiravir, Saquinavir, Remdesivir, Ritonavir, Lopinavir
Abstract: This method is simple and rapid which will make the detection of these potential drugs convenient and efficient and may contribute to additional studies for treatment of COVID-19.

 

No.

Compounds

Concentration (µg/mL)

Retention Time(min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

1

Chloroquine phosphate

0.01

2.16

320.3→247.1

320.3→142.3

2

Favipiravir

0.2

2.54

158.1→141.2

158.1→113.0

3

Saquinavir

0.1

2.66

671.4→570.4

671.4→128.3

4

Remdesivir

0.02

3.14

603.3→ 200.2

603.3→402.2

5

Ritonavir

0.04

4.11

721.3→296.3

721.3→140.3

6

Lopinavir

0.02

4.29

629.4→447.2

629.4→155.3