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UHPLC/MS/MS determination of SSRIs

Method: HPLC-MS
Matrix: Medicine
Application No.: 101936
Componds: Citalopram hydrobromide, Paroxetine hydrochloride, Fluvoxamine maleate, Dapoxetine hydrochloride, Fluoxetine hydrochloride, Sertraline hydrochloride
Columns: Endeavorsil C18 1.8μm , 150x2.1mm
Cat No.: 87004
Sample Pretreatment: Standards: Individual stock solutions of each drugwere prepared in acetonitrile at a concentration of 1 mg/mL.A working solution of the mixture of all six drugs was freshly obtained by dilution of the stock solution with mobile phase.
Conditions: Mobile Phase:
A: 10mM ammonium formate, pH3.5
B: ethanol:methanol:acetonitrile=1:1:2
Gradient: 10 to 20%B in 3min, 20 to 30%B in 2min, 30 to 40%B in 2min, 40 to 70%B in 13min, 70 to 10%B in 0.5min,equilibrate at 10%B for 4.5min.
Flow Rate: 0.2 mL/min;
Injection: 2µL;
Temperature: 25℃
Source: ESI; Ionization Mode: Positive
Publisher: Dikma Technologies Inc.
Keyword: Citalopram hydrobromide, Paroxetine hydrochloride, Fluvoxamine maleate, Dapoxetine hydrochloride, Fluoxetine hydrochloride, Sertraline hydrochloride, UHPLC/MS/MS, SSRIs
Abstract: Six distinct SSRI compounds were separated using a single chromatographic separation in under 17 minutes using a DIKMA Endeavorsil C18 column.

 

No.

Compounds

Concentration (ng/mL)

Retention Time(min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

1

Citalopram hydrobromide

50

11.97

325.4→109.1

325.4→262.2

2

Paroxetine hydrochloride

150

13.66

330.4→192.3

330.4→70.2

3

Fluvoxamine maleate

30

14.83

319.4→71.2

319.4→258.2

4

Dapoxetine hydrochloride

2

15.23

306.3→ 261.1

306.3→157.1

5

Fluoxetine hydrochloride

150

15.45

310.3→148.2

310.3→117.3

6

Sertraline hydrochloride

30

16.46

306.3→275.1

306.3→159.0