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Simultaneous Determination of 13 Antihistamines by a Single HPLC Method

Method: HPLC
Matrix: Medicine
Application No.: 101935
Componds: Pheniramine, Doxylamine, Pyrilamine, Chlorpheniramine, Triprolidine, Desloratidine, Astemizole, Diphenhydramine, Promethazine, Azelastine, Levocetirizine, Terfenadine, Meclizine
Columns: Inspire C18 5 μm 150 x 4.6 mm
Cat No.: 81001
Conditions: Mobile Phase:
A: 0.1% TFA in H2O;
B: 0.02% TFA in MeCN : MeOH = 75 : 25 (v : v)
Flow Rate: 1 mL/min
Injection: 10 µL
Col. Temp: 30 ℃
Detection: UV @ 254 nm

Gradient Program:
Time(min) %A %B
0 88 12
2 88 12
10 80 20
15 50 50
25 20 80
27 20 80
28 88 12
33 88 12
Keyword: HPLC, Antihistamines, Pheniramine, Doxylamine, Pyrilamine, Chlorpheniramine, Triprolidine, Desloratidine, Astemizole, Diphenhydramine, Promethazine, Azelastine, Levocetirizine, Terfenadine, Meclizine
Abstract: High quality separation of these components demonstrates thatInspire C18 column offers rapid separation with outstanding resolution.

 

Elution Order

Compound

Name

Molecular Formula

Molecular

Weight

Stock Solution

C (mg/mL)*

Mixed Std Solution

C (mg/mL)**

Retention Time

(min)

USP Resolution

1

Pheniramine maletate salt

C20H24N2O4

356.4

1.0

12.5

7.2

-

2

Doxylamine succinate salt

C21H28N2O5

388.5

1.0

12.5

7.6

1.9

3

Pyrilamine maleate salt

C21H27N3O5

401.5

1.0

12.5

12.0

22.3

4

Chlorpheniramine maleate salt

C20H23ClN2O4

390.9

1.0

12.5

14.0

12.6

5

Triprolidine hydrochloride

C19H23ClN2

314.9

1.0

12.5

14.2

2.3

6

Desloratidine

C19H19ClN2

310.8

1.0

12.5

14.7

4.7

7

Astemizole

C28H31FN4O

458.6

1.0

12.5

16.8

21.5

8

Diphenhydramine hydrochloride

C17H22ClNO

291.8

10.0

125

17.3

4.3

9

Promethazine hydrochloride

C17H21ClN2S

320.9

1.0

6.25

18.0

6.2

10

Azelastine hydrochloride

C22H25Cl2N3O

418.4

1.0

12.5

18.3

2.1

11

Levocetirizine dihydrochloride

C21H27Cl3N2O3

461.8

1.0

50

19.7

11.1

12

Terfenadine

C32H41NO2

471.7

10.0

125

21.7

13.7

13

Meclizine hydrochloride

C25H30Cl2N2O

445.4

10.0

125

22.0

1.7

* Stock solutions of each compound were prepared separately in a solution of 75:25 (v/v) MeCN : MeOH

** C=Final concentration of each compound in the mixed standards solution. Mixed standards solution was prepared in 0.15% TFA water solution.
Table: 1. Pheniramine;
2. Doxylamine;
3. Pyrilamine;
4. Chlorpheniramine;
5. Triprolidine;
6. Desloratidine;
7. Astemizole;
8. Diphenhydramine;
9. Promethazine;
10. Azelastine;
11. Levocetirizine;
12. Terfenadine;
13. Meclizine