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Simultaneous Determination of 13 Calcium Channel Blockers by LC-MS-MS method

Method: HPLC-MS
Matrix: Medicine
Application No.: 101934
Componds: Amlodipine besylate, Benidipine hydrochloride, Cilnidipine, Clevidipine, Diltiazem hydrochloride, Felodipine, Lercanidipine hydrochloride, Manidipine dihydrochloride, Nicardipine hydrochloride, Nifedipine, Nimodipine, Nitrendipine, Verapamil hydrochloride
Columns: Endeavorsil C18 1.8 μm 100 x 2.1 mm
Cat No.: 87003
Sample Pretreatment: Samples:Stock solution of each CCBswas prepared in methanol at a concentration of 1 mg/mL. Combined working solution was freshly obtained by dilution of stock solution with mobile phase.
Conditions: Mobile Phase: A: 10mM Ammonium acetate
B:Acetonitrile
Gradient: hold35%Bfor1.5min, 35 to 70%B in 3.5min, 70 to 90%B in 9 min, 90 to 35%B in 1min, equilibrate at 35%B for 5 min
Scan mode: ESI+;
Flow Rate: 0.2mL/min;
Injection: 2µL;
Temperature: 30℃
Publisher: Dikma Technologies Inc.
Keyword: Amlodipine besylate, Benidipine hydrochloride, Cilnidipine, Clevidipine, Diltiazem hydrochloride, Felodipine, Lercanidipine hydrochloride, Manidipine dihydrochloride, Nicardipine hydrochloride, Nifedipine, Nimodipine, Nitrendipine, Verapamil hydrochloride
Abstract: All drugs were separated, eluted and analyzed on a 100 x 2.1 mm ID column in 13 minutes.

 

No.

Compounds

Concentration (µg/mL)

Retention Time(min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

1

Amlodipine besylate

0.096

4.5

409.2→294.1

409.2→238.0

2

Benidipine hydrochloride

0.018

9.5

506.2→174.2

506.2→315.2

3

Cilnidipine

0.18

9.0

493.2 →117.2

493.2 →375.2

4

Clevidipine

0.2

8.8

473.2→338.2

473.2→456.2

5

Diltiazem hydrochloride

0.004

4.8

415.2→178.2

415.2→150.2

6

Felodipine

0.14

8.3

384.1→352.1

384.1→338.1

7

Lercanidipine hydrochloride

0.006

12.1

612.3→280.3

612.3→100.2

8

Manidipine dihydrochloride

0.012

10.3

611.3→167.2

611.3→152.2

9

Nicardipine hydrochloride

0.012

7.8

480.2→315.2

480.2→148.2

10

Nifedipine

0.12

6.0

347.2→315.2

347.2→254.4

11

Nimodipine

0.03

7.4

419.2→343.1

419.2→301.1

12

Nitrendipine

0.16

7.0

361.3→329.3

361.3→315.2

13

Verapamil hydrochloride

0.008

5.1

455.4→165.3

455.4→150.2