A Simple Method for Simultaneous Determination of 6 Tricyclic antidepressants by UHPLC/MS/MS

Method:	HPLC-MS
Matrix:	Medicine
Application No.:	101928
Componds:	Nordoxepin hydrochloride, Imipramine hydrochloride, Maprotiline hydrochloride, Amitriptyline hydrochloride, Trimipramine maleate, Clomipramine hydrochloride
Columns:	Endeavorsil C18 1.8μm , 150x2.1mm
Cat No.:	87004
Sample Pretreatment:	Standards: Individual stock solutions were prepared in a mixed solution of acetonitrile and water (8:2 v/v) at a concentration of 1 mg/mL. A working solution of the mixture of all 6 drugs was obtained by dilution of the stock solution with mobile phase.
Conditions:	Mobile Phase: A: 10 mM ammonium formate, pH 3.0 B: methanol:acetonitrile = 4:6 Gradient: hold 30% B for 1.5 min, 30 to 50% B in 1.5 min, 50 to 52% B in 10 min, 52 to 75% B in 1 min, hold 75% B for 1.5 min, 75 to 30% B in 0.5 min, equilibrate at 30% B for 4 min. Flow Rate: 0.2 mL/min; Injection: 2 µL; Temperature: 25℃ Source: ESI; Ionization Mode: Positive
Publisher:	Dikma Technologies Inc.
Keyword:	Nordoxepin hydrochloride, Imipramine hydrochloride, Maprotiline hydrochloride, Amitriptyline hydrochloride, Trimipramine maleate, Clomipramine hydrochloride, Tricyclic antidepressant, UHPLC/MS/MS, Endeavorsil C18
Abstract:	This method, based on UHPLC/MS/MS determination, allowed total separation within 16 min. DIKMA Endeavorsil® 1.8 µm C18 column was used in this method and help compounds achieve good peak shape and resolution. It can be easily applicable to routine analysi