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Separation of Cephalosporin Antibiotics at Different Mobile Phase Conditions

Method: HPLC
Matrix: Medicine
Application No.: 101901
Columns: Inspire C18 5 μm 150 x 4.6 mm
Cat No.: 81001
Conditions: Column: Inspire™ 5 μm C18, 150 x 4.6 mm
Author: Dikma Technologies Inc.
Keyword: Cephalosporin Antibiotics, Cefadroxil, Cefazoline, Ceftazidime, Cefoxitin, Cefaclor, Cefradine, Cephalexin

 

Mobile Phase: MeOH:25 mM phosphate buffer (pH 3) = 20:80  
Flow Rate: 1.0 mL/min  
Temperature: Ambient  
Detection: UV 230 nm  
Sample: 1. Cefadroxil 5. Cefazoline
  2. Ceftazidime 6. Cefoxitin
  3. Cefaclor 7. Cefradine
  4. Cephalexin  

 
Mobile Phase: MeOH:100 mM acetate buffer = 20:80  
Flow Rate: 1.0 mL/min  
Detection: UV 254 nm  
Sample: 1. Cefadroxil  
  2. Cefuroxime 4. Cefoxitin
  3. Cefaclor 5. Cefradine

                                                                                                                                                             
Mobile Phase: MeOH:0.1% TFA in H2O = 30:70  
Flow Rate: 1.0 mL/min  
Detection: UV 230 nm  
Sample: 1. Ceftazidime 5. Cephalexin
  2. Cefadroxil 6. Cefoxitin
  3. Cefazoline 7. Cefradine
  4. Cefaclor