Determination of Phthalate Esters (PAEs) in Water

Method:	SPE
Matrix:	Water
Application No.:	101750
Columns:	ProElut PLS Glass Cartridge, 200 mg / 6 mL 30/pk
Cat No.:	68012G
Sample Pretreatment:	1. Sample purification ProElut™ PLS GLASS 200 mg / 6 mL (Cat#68012G) Condition: 6 mL methyl tert-butyl ether / 6 mL MeOH / 6 mL H₂O Load: Up to 500 mL sample, flow rate ≤15 mL/min Wash: 3 mL 5 % MeOH / H₂O Elute: 3 mL MeOH / 6 mL methyl tert -butyl ether Reconstitute: Evaporate to near dry at 30 ºC, reconstitute to 1 mL with acetonitrile
Conditions:	HPLC method Column: Inspire™ 5 µm C18, 250 x 4.6 mm (Cat#81006) Mobile Phase: A: H₂O, B: MeCN Flow Rate: 1.0 mL/min Temperature: 30 ºC Detection: UV 230 nm Injection Volume: 20 µL
Author:	Dikma Technologies Inc.
Keyword:	Phthalate Esters, PAEs, Inspire, SPE, PLS