Determination of Phenols in Water

Method:	SPE
Matrix:	Water
Application No.:	101748
Columns:	ProElut PLS 60 mg / 3 mL 50/pk
Cat No.:	68003
Sample Pretreatment:	1. Sample preparation 100 mL sample, adjust to pH 2 with H₃PO₄ 2. Sample purification ProElut™ PLS 60 mg / 3 mL (Cat#68003) Condition: 3 mL MeOH:MTBE = 10:90 / 3 mL MeOH / 3 mL H₂O Load: 100 mL sample, flow rate ≤ 5 mL/min Wash: 3 mL H₂O Elute: 3 mL MeOH:MTBE = 10:90 Reconstitute: Evaporate at 40 ºC by N₂, reconstitute to 1 mL with MeCN:H₂O (50:50, V / V) solution
Conditions:	HPLC method Column: Inspire™ 5 µm C18, 150 x 4.6 mm (Cat#81001) Mobile Phase: A: 1% CH₃COOH in H₂O, B: 1% CH₃COOH in MeCN Flow Rate: 1.0 mL/min Temperature: Ambient Detection: UV 280 nm Injection Volume: 20 µL
Author:	Dikma Technologies Inc.
Keyword:	Phenols, SPE, PLS, Inspire