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Determination of H2 Blockers by UHPLC-MSMS Method Using DIKMA Endeavorsil C18 Column

Method: HPLC-MS
Matrix: Medicine
Application No.: 101965
Componds: Nizatidine, Cimetidine, Famotidine, Ranitidine
Columns: Endeavorsil C18-A 1.8μm , 100x2.1mm
Cat No.: 87113
Sample Pretreatment: Standards: Individual stock solutions were prepared in methanol/acetonitrile (1/5) at a concentration of 1 mg/mL. A working solution of the mixture of all 4 drugs was obtained by dilution of the stock solution with mobile phase.
Conditions: Mobile Phase:
A: Ammonium Formate (pH4.7)
B: methanol: acetonitrile = 4: 6
Mobile Phase A: Accurately weigh 23g of formic acid into water, and add water to make the final volume 100mL. Pipette out exactly 15mL of this solution, adjust the pH to 4.7 with 10M ammonium hydroxide. Transfer this solution into a vessel, bring up the volume to 0.5L with water.
Gradient: hold 5% B for 2 min, 5 to 8% B in 13 min, 8 to 60% B in 2 min, 60 to 5% B in 1 min, equilibrate at 5% B for 5 min.
Flow Rate: 0.2 mL/min
Injection: 2 µL
Temperature: 25 ℃
Source: ESI
Ionization Mode: Positive
Publisher: Dikma Technologies Inc.
Keyword: Nizatidine, Cimetidine, Famotidine, Ranitidine, Endeavorsil C18-A
Abstract: This method was establishd by using DIKMA Endeavorsil 1.8 µm C18-A column. With simple mobile phase and a gradient procedure, these four compounds can be eluted from the column within 15 mins.

 

 

No.

Compounds

Concentration (µg/mL)

Retention Time (min)

MS→MS1 Ions

(Precursor→Product 1)

MS→MS2 Ions

(Precursor→Product 2)

1

Nizatidine

0.1

9.15

332.4→155.1

332.4→131.1

2

Cimetidine

0.1

12.70

253.3→159.2

253.3→95.0

3

Famotidine

0.1

13.48

338.3→189.1

338.3→259.3

4

Ranitidine

0.1

14.05

315.4→176.2

315.4→130.0